学 术 报 告  

　　报告题目:  Discriminating Bases by stretching Double-Stranded DNA in a Solid-State Nanopore 

　　报告人:   Dr. Deqing Wang 

　　主持人：Dr. Dexing Zhang 

　　时   间：2013年11月1日,上午10：30－11：30 

　　地   点：中国科学院北京基因组研究所一楼报告厅（北郊园区东门内） 

　　Abstract： 

　　It is now possible to slow down and trap a single molecule of double-stranded DNA (dsDNA) in a solid-state nanopore that is smaller in diameter than the double helix.  This technique relies on a thorough understanding of the electromechanics of DNA in a solid-state nanopore <3.0nm in diameter.  By applying a voltage larger than the threshold for translocation, dsDNA can be forced to translocate through the pore.  Once a current signature associated with a translocating molecule is detected, the electric field in the pore is switched to a value below the threshold, substantially reducing the translocation velocity from 1bp/10ns to >1bp/17μs. We can discriminate the difference between base pairs (AT/CG) with electrical current by trapping the biotinylated double-stranded DNA with streptavidin inside nanopores. 

　　Biography: 

　　Deqiang Wang received the B.S. in the Department of Electrical Engineering from University of Jilin, Changchun in 2001 and the Ph.D degree from the Institute of Microelectronics, Chinese Academy of Sciences, in 2006. Currently, he is a postdoctoral research associate in IBM T.J Watson research center, NY. His main research intersest is single molecule nanopore detection and nanopore-based DNA sequencing. His research activities also include e-beam, X-ray lithography, micro and nano fabrication. He coauthored over 30 peer reviewed journal papers and conference presentations. He has about 18 granted and pending US patents. 

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